![](/files/sbc/styles/full_width/public/imce/microscopy/Microscopy-Nikon-A1R_02.jpeg?itok=x9iPbiqX)
Overview
- Laser scanning confocal with 4 PMT detectors
- Optional 32 channel detector for virtual filters or spectral unmixing
- Widefield camera available for image overviews
Specifications
- Inverted Ti Nikon microscope
- Nikon XYZ Motorized stage with perfect focus module for Z stability
- Environment chamber with temperature control and CO2 enrichment
- Nikon A1-SHR High Speed Scan Head & Controller (Resonant Scanner)
- Nikon NIS Elements Acquisition Software
- 40x water immersion objective for deeper tissue imaging
- Confocal mode:
- 4 lasers and 4 Standard Photomultipliers (PMT) detectors
- Galvonometer scanners up to 4096 x 4096 pixels per field
- Resonant scanners for fast 512x512 acquisition at 30 frames/sec
- Optional 32 channel spectral detector (wavelength range: 400-770 nm)
- Optional transmitted light detector
- Widefield mode
- Standard epifluorescence with halogen excitation lamp
- Andor Clara interline transfer CCD
Fluorescent Filters and Lasers
Name | Set | Std dyes | Cy series | Alexa | Ex | Em |
---|---|---|---|---|---|---|
DAPI | Widefield | DAPI/Hoechst/BV421 | 395 | 458 | ||
FITC | Widefield | FITC | Cy2 | AF488 | 470 | 535 |
TRITC | Widefield | TRITC/TxRed | Cy3/Cy3.5 | AF546/594 | 550 | 625 |
Cy5 | Widefield | APC | Cy5/Cy5.5 | AF647 | 640 | 675 |
405 Laser | Std Confocal | DAPI/Hoechst/BV421 | 405 | 440 | ||
488 Laser | Std Confocal | FITC | Cy2 | AF488 | 487 | 525 |
561 Laser | Std Confocal | TRITC/TxRed | Cy3/Cy3.5 | AF546/594 | 561 | 625 |
640 Laser | Std Confocal | APC | Cy5/Cy5.5 | AF647 | 637 | 675 |
405 Laser | 32 Chan Conf | DAPI/Hoechst/BV421 | 405 | Tuneable | ||
488 Laser | 32 Chan Conf | FITC | Cy2 | AF488 | 487 | Tuneable |
561 Laser | 32 Chan Conf | TRITC/TxRed | Cy3/Cy3.5 | AF546/594 | 561 | Tuneable |
640 Laser | 32 Chan Conf | APC | Cy5/Cy5.5 | AF647 | 637 | Tuneable |
Objectives
Objective | OIL | Corr | WD mm | Z DOF µm |
---|---|---|---|---|
4x/0.2 | Plan Apochromat | 15.70 | 21.88 | |
10x/0.30 | Plan Fluor Ph1 DLL | 16.00 | 8.28 | |
40x/1.15 | Water | LWD Apochromat Lambda S | 0.59 - 0.61 | 0.74 |
60x/1.40 | Oil | Plan Apochromat VC DIC N2 | 0.13 | 0.54 |