Nikon A1R Ultra-Fast Spectral Scanning Confocal Microscope

Overview

  • Laser scanning confocal with 4 PMT detectors
  • Optional 32 channel detector for virtual filters or spectral unmixing
  • Widefield camera available for image overviews

Specifications

  • Inverted Ti Nikon microscope
  • Nikon XYZ Motorized stage with perfect focus module for Z stability
  • Environment chamber with temperature control and CO2 enrichment
  • Nikon A1-SHR High Speed Scan Head & Controller (Resonant Scanner)
  • Nikon NIS Elements Acquisition Software
  • 40x water immersion objective for deeper tissue imaging
  • Confocal mode:
    • 4 lasers and 4 Standard Photomultipliers (PMT) detectors
    • Galvonometer scanners up to 4096 x 4096 pixels per field
    • Resonant scanners for fast 512x512 acquisition at 30 frames/sec
    • Optional 32 channel spectral detector (wavelength range: 400-770 nm)
    • Optional transmitted light detector
  • Widefield mode
    • Standard epifluorescence with halogen excitation lamp
    • Andor Clara interline transfer CCD

Fluorescent Filters and Lasers

Name Set Std dyes Cy series Alexa Ex Em
DAPI Widefield DAPI/Hoechst/BV421     395 458
FITC Widefield FITC Cy2 AF488 470 535
TRITC Widefield TRITC/TxRed Cy3/Cy3.5 AF546/594 550 625
Cy5 Widefield APC Cy5/Cy5.5 AF647 640 675
             
405 Laser Std Confocal DAPI/Hoechst/BV421     405 440
488 Laser Std Confocal FITC Cy2 AF488 487 525
561 Laser Std Confocal TRITC/TxRed Cy3/Cy3.5 AF546/594 561 625
640 Laser Std Confocal APC Cy5/Cy5.5 AF647 637 675
             
405 Laser 32 Chan Conf DAPI/Hoechst/BV421     405 Tuneable
488 Laser 32 Chan Conf FITC Cy2 AF488 487 Tuneable
561 Laser 32 Chan Conf TRITC/TxRed Cy3/Cy3.5 AF546/594 561 Tuneable
640 Laser 32 Chan Conf APC Cy5/Cy5.5 AF647 637 Tuneable

Objectives

Objective OIL Corr WD mm Z DOF µm
4x/0.2   Plan Apochromat 15.70 21.88
10x/0.30   Plan Fluor Ph1 DLL 16.00 8.28
40x/1.15 Water LWD Apochromat Lambda S 0.59 - 0.61 0.74
60x/1.40 Oil Plan Apochromat VC DIC N2 0.13 0.54