Nikon Spinning-Disk Confocal Microscope with TIRF module

Overview

• Good widefield camera with LED illuminator
• Variable angle TIRF exication arm
• Spinning disk confocal with small field-of-view for live-cell imaging

NOTES

• This is an older (1st gen) spinning disk confocal scanning head with a small field of view
• The confocal unit is not intended for scanning large samples
• COMING SOON: We will have a modern Nikon spinning disk confocal available when the Building 68 (Bio) core extension opens in Spring 2025

Specifications

• Inverted Ti Nikon microscope
• Nikon XYZ Motorized stage with pefect focus module for Z stability
• Environment chamber with temperature control and CO2 enrichment
• Agilent dual-fiber laser box can be directed either to the confocal head or TIRF illuminator
• Nikon NIS Elements Acquisition software
• Confocal Mode
  • McBain-Yokogawa CSU-10 (1st gen) spinning disk confocal head with fiber optic input
  • Retiga cooled interline transfer CCD camera
  • Fast-switching filter wheel
• Widefield and TIRF Mode
  • Spectra X 7 wavelength solid state LED illuminator
  • Andor Zyla 4.2 sCMOS sensitive cooled camera (back-illuminated)
  • Motorized variable angle TIRF for adjustment of TIRF penetration depth
  • TIRF uses same excitation lasers as confocal head

Fluorescent Filters and Lasers

Objectives

*Awaiting Repair